Tuesday, July 23, 2019
The Design Procedure for Fire Suppression Systems Research Paper
The Design Procedure for Fire Suppression Systems - Research Paper Example The team must unearth whether the process is a chemical synthesis or merely a mixing process. The team must also ascertain the pressure and temperature conditions of the raw materials. Accordingly, the project management team must proactively assess and understand the duration of the process and the role of each operation staffs. The team must also consider such factors as the existence of purification or drying step. The mechanism of packaging and storage, as well as storage of finished products, call for attention. In addition, it is significant to gain an understanding of the unit operation and process within the facility. Such information results from the study of process and instrumentation diagrams and narrative process description. Finally, there must be a complete list of hazardous materials. The list must be accompanied by chemical names, container type and size, and solution concentrations. Various codes and criteria particularly on the industrial and chemical plants provides critically relevant and essential information on the fire suppression and detection designs. FPE identifies the applicable codes as well as standards for a given industrial process and assists particular facility operators to fathom their respective and appropriate to particular situations. The codes and standard are in line with the understanding of engineering benchmarks for manufacturing procedures and facility design. The design and industrial operation also rests on the underwriting requirements. The FM Global datasheets are critical in determining the requirements for a range of industrial facilities that shapes the fire suppression designs. Underwriting standards presents valuable data against the hazards further impacting the fire suppression designs. Finally, the design process for fire suppression richly rests on the technical information from FM Global Datasheets 7-44
Monday, July 22, 2019
To Watch the Faces of the Poor Essay Example for Free
To Watch the Faces of the Poor Essay Charles Cunningham, in ââ¬Å"ââ¬Å"To Watch the Faces of the Poorâ⬠: Life Magazine and the Mythology of Rural Poverty in the Great Depression (1999),â⬠details how Life magazine describes the agrarian poverty in 1930ââ¬â¢s due to Great depression, ridiculing the poor of whites by showing photographs without enough explanation of this poverty. Exclusion of explaining the cause of poverty in agriculture possibly leads to these ââ¬Å"White poorâ⬠as ââ¬Å"worthyâ⬠poor, who are not inherently inferior, but are victims of nature and geography. Tom Delph-Janiurek, in his article of ââ¬Å"Sounding Gender(ed): Vocal Performances in English University Teaching Spaces,â⬠states that gendered voices are performed through repeated stylization of bodies, having connection with gendered and sexualized identities. Also, he argues that voice have a geography shaped by how discourses change across different types of space. Both these authors discuss how pre-held notions construct the distorted bias of poverty in race and gendered voices respectively. According to Cunningham, by showing many pictures of poor white in U. S. rural regions such as Oklahoma, Montana, Arkansas, and Dakota, Life magazine effectively aroused sympathy from readers of Life magazine who subsequently thought their poor condition was mainly attributable to misfortune. This limited a reasonable explanation of poverty of ââ¬Å"worthy poor. â⬠The readers of this magazine only saw the pictures of untidiness, biological unfitness, and sloth of the poor white, so only consider that they were handicapped sufferer by geographical conditions. The direct factor of this economic crisis, capitalism itself, was never involved or even considered as the cause of the poor condition in Life magazine. Thus, the readers of Life did not expand themselves as unworthy to be poor, and think of becoming destitute as a result of economic crisis. In Delph-Janiurekââ¬â¢s discussion of voices, although gendered dualism of voices seems obvious having distinctive characteristics in a certain way, voices actually have geography with involving the production and interpretation of them in particular ways within different kinds of spaces. It means that voices have much more unique ways of interpretation than the vocal performance of roles and identities. Audiences themselves grasp the context of speaking according to specific interactional spaces and geography. The understanding of talk is not only related to binary gendered voices, but also to surroundings and desired narratives. For example, when performing in drama, students would be required to relinquish one kind of vocal and adopt another to alter unenthusiastic, unemotional voices of heterosexual masculinity to more emotional theatrical voices through a process called ââ¬Å"drag. Along with dragged voices, lesbian and gay voices can be performed vocally in ways that mimic the gender dualism. Some gay men can use extreme versions of ââ¬Å"womenââ¬â¢s voicesâ⬠to act ââ¬Å"campâ⬠identities, or vice versa. Taken together, mythology of poverty with hidden crucial factor of economical crisis and various forms of vocal performance would result in abs olutely different interpretation by readers or audiences.
Sunday, July 21, 2019
English Literature Abstract Expressionism Movement
English Literature Abstract Expressionism Movement Abstract Expressionism Movement While it was generally conceded that Abstract Expressionism was spent, superseded by the rise of minimalism and Pop art in the early 1960s, Gustans work after 1968 signaled the end of the Idealism that had driven the movement. Some artists such as Newman, cited in (the book); had continued to question whether the New York School had existed at all, stating as late as 1965 that: there was never a movement in the conventional sense of a style, but a collection of individual voices. That is why to talk of the movement being dead is ridiculous. But I have found that this book although it in general, covers the factual information necessary to form a coherent opinion, it nevertheless, rambled, leaving the reader with limited knowledge that conceptualized a clear understanding. In essence, I found it hard to read, and that in some places it also repeated facts. Therefore, I gave serious consideration to the other listed books on the same subject, but further extended my research to more generalist books that covered the history of art. In returning to the text, Abstract Expressionism by Anfam (1990), which offers an albeit extended and sometimes incoherent journey through this subject, we can at least in part find some nuggets of information that carefully explain and discuss some of the wider concepts of abstract expressionism. But, to find the most sensible and coherent facts, I had to delve deeply into this volume, which at best, left the reader often lost and in many cul-de-sacs, where opinions were losing the force and direction of their meaning because of very poor signposting techniques used by the author. At best, the useful and expressive textual details were lost and to re-address this and to have some coherent understanding of the importance and standing of abstract expressionism, I had to cross reference many of the themes in this book, to other texts, which at best, allowed me to form an holistic and guided academic opinion on the movement. Therefore, this book review will provide some carefully constructed opinions guided by the chosen text, but, more fully engaged and directed by the supplementary reading undertaken. REVIEW Anfam (1990) in his work, Abstract Expressionism, suggests that there was a group of artists and like-minded people, who founded the movement. In doing so, he cites aspects of the modern history of the USA, in particular, the depression. In widening his argument, he also reveals some of the many parallels with the unrest within the French expressionism movement and equally rambles on to try to offer some formative cross referencing with the many expressive styles of other movements of the era. This discursive and sometimes lively debate is hampered by a clear lack of coherence, it leaves as stated in my introduction, the reader in many a cul-de-sac, with a real battle of wits to find some real and meaningful information that clearly nails down the subject of abstract expressionism in its diluted forms. The work of Pollock is often cited in the text to explain how artists use their work to show feelings and emotions, in part this aspect is explain and discussed in fine details that allow the reader to make some careful informed opinions. In particular Anfam (1990) tries his best to offer through he opening arguments contained in the introduction to the text, a socio-historical foundation for his later discussions, for example: before the second World War opens perspective that enfold the present. In microcosm we might compare of the western political power and culture after 1945. This interesting and meaningful conceptualization, offers some cohesion to the ongoing debate, setting the social and historical scene is crucial, in that, the World had just been through the most devastating periods of modern history, leaving a bereavement and albeit soulless notion of loss that impacted upon all aspects of society. From this vacuous void, came the development of what we know as modern sustainable artistic talents that in part, were able to bring a real flavour of expressing emotions through the wonderful and many diverse styles that were emerging out of this vacuum. The examples could be many, but for Anfram (1990), it is expressed in his notion that modern companies, that is commercial enterprises, became an everyday occurrence, in that, through abstract expressionism, revealed the founding symbols of modernity. Historically according to Anfram (1990) it is worthy of note, that the western world, in particular, the USA and UK, emerged from the devastation of World War I (WWII). But, more prudently, the USA emerged as the creditor, backer and in the main banker to the countries devastated by the war. A factor to only repeated some decades later. This often if not often obscured action by the USA, brought about the fundamental and sustainable changes in consumerism, fueled by the consistency and often aggressive expansion of industrialization across the Western World. Bringing about what we cited today as modern consumerism. However, what is also of crucial note, in particular for engaging artists, is the rise in the popular movements of mass culture and modern technology. Which impacted upon a world that had suddenly started to grow up out of the vacuum of aggressive conflict. What is abundantly clear from the history of art at this time, is that, Abstract Expressionism, although crisp and modern in its outlook, coupled with its diversity, it nevertheless, lacked clear shape, which was in the main, fuelled by the ever pessimistic negative vibrations that the depression and war years had invoked. Shaking off this cloud of pessimism would take some serious shock waves to kick start a new an meaningful movement that signaled the beginnings of what we now know as abstract expressionism (Gombrich 1984; Levey 1968). The early years of Abstract Expressionism, according to most theorist, (Balken 2005; Polcari 1999; Stangos (ed) 1981 Pickeral 2007); appeared to lacked shape against both this over whelming sense of optimism and yet another more negative vein, which had been intensified by the depression period. The clarity of the pre-depression period as seen in the artistic work of Charles Burchfield and Edward Harper, was fuelled by the onset fear that depression would bring, in so doing, their art revealed the styles that poignantly marked this period. This notional and albeit national fear is expressed in many of the texts used for this review, but, equally cited and noted in the core text by Anfram (1990). Interestingly his work reaches a watershed in which we have an interesting and yet revealing discourse about the artists of the period. However, it is not difficult to find his particular favorite. He in particular, cites in his discourse, the life and work of Pollock, citing the many and meaningful paths that his life took and how this impacted upon his revealing and yet sometimes provocative understanding of the thematic aspects of depression, one might even say, it is depressive and yet expressive art! The critique of Pollock by Anfram (1990), is one of the lighter parts of this text, in that we are provided with a snapshot history of this artist, his struggles and battles to live and develop as an expressive artist. In particular, we find a detailed history of his ancestry, to include the occupational aspects, upbringing and lifestyle of this Jewish family, set against a backdrop of his homeland. But, the most revealing facts are the way in which his argument convincingly places the links between Pollock and other artists like: Guston and Benton. This what could be seen a meeting of minds, signaled in part the foundational beginnings of Abstract Expressionism as we know style it. Some of the facts in the text are fascinating, in that, how life paths merge in the meeting of key personalities who make such significant marks on the landscape of art. For example: Anfram (1990) reveals how Pollock met Guston prior to his enrolment in 1930 at the Art Student League in New York, under the direction of Benton. This in itself to the writer seems uncanny and yet perfectly placed in that what came out of the merging of these personal journeys is a breath taking step in the history of modern art. What is fundamentally clear, is that Pollocks own personal history and upbringing reveals a man who had engaged in and fully experienced the construction of depression in all its many sometimes stark and conflicting facets. His journey is clearly a meaningful aspect of the work of Anfram (1990), who lyrically expresses great interest in ensuring his readers are informed of the important fact about Pollock, but, most importantly, showing how this upbringing, made the man and ultimately the artist and his style. It is worth noting some of those facts. Pollock knew all about the depression as his family had to flea the Baltic states, as a Jewish family like that of his peers, Rothko Siskind, he would have to settle in another land to ensure that it would not be so difficult for him to explore his artistic talents, and so his coming to america was a significant step in the story of Abstract Expressionism. For him to have remained in such a stark country that was consistently engaged in political unrest would have changed the focus of this concept of art as we now know it. It is also fairly obvious that Anfram (1990) has a passion for Guston, in that he equally sets his reader on a research quest from his revealing treatment about this artist. He states that Guston has known all about oppression and in so doing, in equal measure his knowledge of the sectarian movement, known as the Klu Klux Klan, whose membership in the 1920 s prior to the 1930s depression topped a staggering five million. If we place both Pollock and Guston alongside each other patterns emerge, in that, Anfram (1990) clearly wants his readers to make some informed opinion and clarity towards how expressionism was founded. Perhaps the missing signposting of his book is in part due to his stop, think and link approach. If we do just that, we can find that, it does not take long to place the constructions of depression in Pollocks background, and the oppression, noted in Guston background bring together the emotional expressive constructions that are revealed by both artists through their conceptualized notion of what they seen and perceive as Abstract Expressionism, that allows the artist in a sometimes breathtaking manner, to engage the viewers of such works, to think outside the box, in other words, see beyond the form, revealing the pain and emotions that are depression and oppression (Gombrich 1984). Anfram (1990) continues his revealing and yet peppered approach to this important movement with his critique of the 1930s depression, treating his readers to, in part have some understanding of the stuggle and battles fought and won by many of the artists of the period. It is obvious that no artist had had an easy journey, on the contrary, the movement had, brought the conceptualization of what is art kicking and screaming into a more coherent and modern take, that allowed for at best, the artist to think and construct outside the box, in so doing, draw in a more liberalized breath of fresh air in to the stifling world of the artist. As the development of Abstract Expressionism, took root, according to Anfram (1990) the 1950s saw a merging of like minded artists, who for the first time were able to finally break with the traditional constructions that had shackled art. This is poignantly revealed in the citation of the New York, Studio 35 conference, in which what is now know as a contradictory discussion allowed for artist to finally discuss and exchange ideas about how each participant broke with the sometimes constrictive traditional media, techniques and constructs that embedded art at its core. The outcome being, what can only be described as a breach of fresh air. What came out of this merging of minds, were a constituted advance in painting, sculpture and even photography. Questions that once thwarted artists were now in vogue and could be explored without constriction: Should it be Abstract or should it be representational? Would foreigner influence vitiates or even strengthens it? Would expression of feeling of the reality of the medium and of everyday? These were the fundamental poignant questions that came out of it in the 1930s, without which we would never have seen to a certain extent, Cubism, Social Realism to name but two aspect of what can be perceived as art outside the box. Briefly it is worth noting some of the constructs that came forward. At this period of time cubism was the normal Art like that of Bombardnent, who was fully engaged in Realism and Cubism. During the 1941 to 1943 period, Clement Greenburg edited an arts magazine, and befriended serial Abstract Expressionists and would cite them as a representation of a new American Art This coupled with the fact that Gorky in his remarks that Social Realism was the form of poor art for the poor people, where as the writers associated with the magazine Parisian Review, which was funded in the 1934 on the Stalinist times, would express diverse and yet carefully constructed remarks that fuelled the debate that has informed and constructed Abstract Expressionism'(Anfram 1990; Gombrich 1984). The text become repetitive in parts in that, it consistently appears to reinforce the facts presented to the reader for example. It over emphasizes the foundations and founder of what is seen to be modern abstract expressionism. Although what is helpful is the authors pick of the best examples of strong abstract expressionists, like: Newman, Pollock, Greenberg, Hofmann, De-Kooning, Gorky, Graham and Krasner. Pollocks artistic methods are carefully followed and explained, in that is it most obvious to the reader that the author is absolutely enthralled with his work. But, interestingly, he again peppers his work with historical facts like, the fact that: the Boston inst of modern Art switched its titular modern to contemporary and the senator George Dondero, denounced non-academic twenty century painting as communist subversion, so Abstraction once again acquired the radical aura that it held for a previous generation (Anfram 1990). Abstract Expressionism always resisted a single collective identity based on style, theories or even social ties. In comparison to Abstract Expressionism and the modern experience by Stephen Polcari 1999, The book under review, is easier to read and one can understand, and can even in formality of the introduction fined relevant and factual information, from which opinions can be formed, for example: there were few Americans Artists, critics, or even modern art historians of the 1950s and early 1960s who did not cut their teeth on it From it very beginnings, Abstract Expressionism has been interpreted in the light of the cultural and intellect of the 1950s artists and theorist, who understood Abstract Expressionism as Harold Rosenbergs typically defined action painting. According to which the artist theatrically expressed their personal anguish on a blank canvas, with little attention to form, style, or subject matter, for Rosenberg, painting was an autobiographical act of self-creation in the everyday world, and the expression of individual personality. Therefore, it can be viewed that the way that Abstract Expressionism looks to the writer, as if, in typical fashion, you just simply splash your thinking and feelings by just letting yourself go and just splurge it onto a canvas and with such painting, you could in fact come up with a not dissimilar work of art. But, never forgetting in the typical construction of this style, to let the paint do all the work with the brushes. In a shape and form of it own. The reviewed book then goes onto explaining where the movement of abstract expressionism came from and what earlier art movements helped it on its way, this gives any reader far better understanding and layout of what the movement conceptualizes. In the words of the author:- giving the art the best pedigree by linking it with impressionism, Cubism and Surrealism this formalist approach focused on the Abstract expressionism stylistic evolution while proposing for them an exclusive concern with the formal questions: purifying the medium, squeezing out illusionism, and remaking space and a optical rather than tactile . CONCLUSION Therefore, it can be attested, that many of the movements discussed as impacting on abstract expressionism are all clearly linked, one could even say that Surrealism been linked to pagan art, in that, they are all from the same family breeding. So therefore conceptual pagan art would notably come under all of these modernist art movements, even socialism can be placed into this context even if it does have its foundations in Russian history. What is interesting is that, all are from a similar systemic background; perhaps, it could be reviewed that they should all be grouped in one mass movement, like that of the early 20th century movement created through, mass observation. But, what is fundamentally of note, in that, art moves and changes constantly, and for any artist, theorist or aspiring artist, it can be very hard to keep up with it evolution. To note this: the big bang theory of abstract expressionism, as ROBERT ROSEBBERG was wittily called it, argued that this abstract work erupted and suddenly. Fully-grown from virtually no where. This perhaps flippant academic remark, makes it sound that abstract expressionism, grew up to quickly upstage, more formal and founding artistic concepts and movements. But, as one can see in the rise of Surrealism, it took between 10-20 years to mature, so inevitably so will this movement, as it has and continues to do so, with ever evolving creations, like the dynamic art being explored in Paganism. Therefore, it can be concluded that art evolves, movements come and go, but the strength and breadth of the artist will always be creatively and in vogue. BIBLIOGRAPHY ANFAM; D. (1990) Abstract Expressionism Thames Hudson BALKEN; D. (2005) Movement In Modern Art Abstract Expressionism TATE Publishing GOMBRICH; E. H. (1984) The History of Art Fourth Edition; Phaidon. Oxford. LEVEY; M. (1968) A History of Western Art Thames Hudson. London. POLCARI; S. (1999) Abstract Expressionism And The Modern Experience UNIVERSITY OF CAMBRIDGE STANGOS; N. (1981) Concepts of Modern Art Thames Hudson
Alcohol Dehydrogenase in Plant Response to Drought
Alcohol Dehydrogenase in Plant Response to Drought 1. Introduction Plant growth and productivity is adversely affected by natures wrath in the form of various abiotic and biotic stress factors (e.g. salinity, low temperature, drought, and flooding heat, oxidative stress and heavy metal toxicity). All these stress factors are a menace for plants and prevent them from reaching their full genetic potential and limit the crop productivity worldwide. Abiotic stress is the principal cause of crop failure, decrease average yields for most major crops by more than 50% (Bray, 2000) and causes losses worth hundreds of million dollars each year. In fact these stresses, threaten the sustainability of agricultural industry (Shilpi, 2005). Environmental degradation and climate change have become severe global problems because of the explosive population increases and industrialization in developing countries. To solve this problem, one of the keys is plant biotechnology based on physiology of crop, plant biochemistry, genomics and transgenic technology. This is becoming more and more important for molecular breeding of crops that can tolerate droughts. For this technology, we need to understand plant responses to drought stress at the molecular level. For agricultural and environmental sustainability, it is important to breed or genetically engineer crops with improved stress tolerance. The identification of key genes and that gene can be used directly for engineering transgenic crops with improved drought tolerance. Although a number of candidate genes have been identified in recent years, only very few have been tested in functional assays for a beneficial effect on drought tolerance. In order to assess gene function directly in plant suffering from abiotic stress caused by the drought, proved to be useful. Analysing the functions of these genes is critical for understanding of the molecular mechanisms governing plant stress response and tolerance, ultimately leading to enhancement of stress tolerance in crops through genetic manipulation. In this study, this will be used for overexpression of genes as well as for induced gene silencing, by using GATEWAY technology. A comprehensive investigation of Adh and Pdc induction and the determination of ethanol production during stress treatments would provide valuable information on how ethanol involved in the response to limited water condition. 2. Literature review 2.1. What is stress? Stress in physical terms is defined as mechanical force per unit area applied to an object. In response to the applied stress, an object undergoes a change in the dimension. Biological term is difficult to define in the plant stress. A biological condition, which may be stress for one plant may be optimum for another plant. The most practical definition of a biological stress is an adverse force or a condition, which inhibits the normal functioning and well being of a biological system such as plants (Jones et al., 1989 ) 2.2. Stress signalling pathways The stress is first perceived by the receptors present on the membrane of the plant cells , the signal is then transduced downstream and this results in the generation of second messengers including calcium, reactive oxygen species (ROS) and inositol phosphates. These second messengers, further modulate the intracellular calcium level. This Ca2+ level is sensed by calcium binding proteins, Ca2+ sensors. These sensory proteins then interact with their respective interacting partners often initiating a phosphorylation cascade and target the major stress responsive genes or the transcription factors controlling these genes. The products of these stress genes ultimately lead to plant adaptation and help the plant to survive the unfavourable conditions. Thus, plant responds to stresses as individual cells and synergistically as a whole organism. Stress induced changes in gene expression in turn may participate in the generation of hormones like ABA, salicylic acid and ethylene. The various stress responsive genes can be broadly categorized as early and late induced genes. Early genes are induced within minutes of stress signal perception and often express transiently. In contrast, most of the other genes, which are activated by stress more slowly, i.e. after hours of stress perception are included in the late induced category. These genes include the major stress responsive genes such as RD (responsive to dehydration)/ KIN (cold induced)/COR (cold responsive), which encodes and modulate the LEA-like proteins (late embryogenesis abundant), antioxidants, membrane stabilizing proteins and synthesis of osmoly tes. 2.3. Drought stress Among all abiotic stresses, drought is one of the most serious problems for sustainable agriculture worldwide. The adverse effect of drought stress is reductions in yield as reported in crops such as rice (Oryza sativa) (Brevedan and Egli, 2003), wheat (Triticum aestivum) (Cabuslay et al., 2002), soybean (Glycine max) (Kirigwi et al., 2004), and chickpea (Cicer aerietum) (Khanna-Chopra and Khanna-Chopra, 2004). The adaptive responses to drought must be coordinated at the molecular, cellular, and whole-plant levels. These conditions induce dehydration of plant cells, which may trigger physiological, biochemical and molecular responses against such stresses (Shinozaki and Yamaguchi, 1996). Water deficit is a complex of responses, which depends upon severity and duration of the stress, plant genotype, developmental stage, and environmental factors providing the stress. Yield losses due to drought are highly variable in nature depending on the stress timing, intensity, and duration. Although, different plant species have variable thresholds for stress tolerance, and some of them can successfully tolerate severe stresses and still complete their life cycles, most cultivated crop plant species are highly sensitive and either die or suffer from productivity loss after they are exposed to long periods of stress. It has been estimated that two-thirds of the yield potential of major crops are routinely lost due to unfavourable growing environments ( Shilpi, 2005 ). Plants have evolved a number of strategies to severe drought. These include escape strategies such as avoidance (flowering, deep rooting, enhanced water uptake efficiency, or reduced water loss) as well as tolerance mechanisms. Reduced shoot growth and increased root development could result in increased water absorption and reduced transpiration, thereby maintaining plant tissue water status. In addition to such avoidance mechanisms, plant responses to water shortages can involve changes in biochemical pathways and expression of genes encoding proteins that contribute to drought adaptation. The proteins could be enzymes involved in the synthesis of osmolytes, antioxidants, or hormones such as ABA and others. Such changes can bring about drought tolerance, whereby plants continue to function at the low water potentials caused by water deficit (Hall, 1993). A central response to water deficit is often increased synthesis of ABA, which in turn induces a range of developmental (avoidanc e) and physiological or biochemical (tolerance) mechanisms. There is an ongoing debate as to whether the exploitation of avoidance or tolerance mechanisms should be the focus of plant breeding programmes. However, it appears likely that the exploitation of tolerance mechanisms may be more promising for the stabilization of crop yield under severe drought conditions (Araus et al, 2002). An assortment of genes with diverse functions are induced or repressed by these drought stresses (Bartels and Sunkar, 2005; Yamaguchi and Shinozaki, 2005). Drought tolerance has been shown to be a highly complex trait, regulated expression of multiple genes that may be induced during drought stress and thus more difficult to control and engineer. Plant engineering strategies for abiotic stress tolerance rely on the expression of genes that are involved in signaling and regulatory pathways (Seki and Shinozaki, 2003) or genes that encode proteins conferring stress tolerance (Wang, 2004) or enzymes present in pathways leading to the synthesis of functional and structural metabolites. Current efforts to improve plant stress tolerance by genetic transformation have resulted in several important achievements; however, the genetically complex mechanisms of abiotic stress tolerance make the task extremely difficult. 2.3.1 Physiological and biochemical responses of drought Physiological and biochemical changes at the cellular level that are associated with drought stress include turgor loss, changes in membrane fluidity and composition, changes in solute concentration, and protein and protein-lipid interactions (Chaves et al,2003) . Other physiological effects of drought on plants are the reduction in vegetative growth, in particular shoot growth. Leaf growth is generally more sensitive than the root growth. Reduced leaf expansion is beneficial to plants under water deficit condition, as less leaf area is exposed resulting in reduced transpiration. Many mature plants, for example cotton subjected to drought respond by accelerating senescence and abscission of the older leaves. This process is also known as leaf area adjustment. Regarding root, the relative root growth may undergo enhancement, which facilitates the capacity of the root system to extract more water from deeper soil layers. Plant tissues can maintain turgor during drought by avoiding dehydration, tolerating dehydration or both (Kramer,1995). These forms of stress resistance are controlled by developmental and morphological traits such as root thickness, the ability of roots to penetrate compacted soil layers, and root depth and mass (Pathan, 2004). By contrast, adaptive traits, such as osmotic adjustment and dehydration tolerance, arise in response to water deficit . Reduction of photosynthetic activity, accumulation of organic acids and osmolytes, and changes in carbohydrate metabolism, are typical physiological and biochemical responses to stress. Synthesis of osmoprotectants, osmolytes or compatible solutes is one of the mechanisms of adaptation to water deficit. These molecules, which act as osmotic balancing agents, are accumulated in plant cells in response to drought stress and are subsequently degraded after stress relief (Tabaeizadeh ,1998). 2.3.2 Molecular responses Studies on the molecular responses to water deficit have identified multiple changes in gene expression. Functions for many of these genà ¨ products have been predicted from the deduced amino acid sequence of the genes. Genes expressed during stress are anticipated to promote cellular tolerance of dehydration through protective functions in the cytoplasm, alteration of cellular water potentia1 to promote water uptake, control of ion accumulation, and further regulation of gene expression. Expression of a gene during stress does not guarantee that a gene product promotes the ability of the plant to survive stress. The expression of some genes may result from injury or damage that occurred during stress. Other genes may be induced, but their expression does not alter stress tolerance. Yet others are required for stress tolerance and the accumulation of these gene products is an adaptive response. Complex regulatory and signaling processes, most of which are not understood, control the expression of genes during water deficit. In addition to induction by stress, the expression of water-deficit-associated genes is controlled with respect to tissue, organ, and developmental stage and may be expressed independently of the stress conditions. The regulation of specific processes will also depend upon the experimental conditions of stress application. Stress conditions that are applied in the laboratory may not accurately represent those that occur in the field. Frequently, laboratory stresses are rapid and severe, whereas stress in the field often develops over an extended period of time ( Radin, 1993). These differences must also be evaluated when studying the adaptive value of certain responses. The function of the gene products and the mechanisms of gene expression are intertwined, and both must be understood to fully comprehend the molecular response to water deficit. 2.4. Function of water-stress inducible genes Genes induced during water-stress conditions are thought to function not only in protecting cells from water deficit by the production of important metabolic proteins but also in the regulation of genes for signal transduction in the water-stress response . Thus, these gene products are classified into two groups. The first group includes proteins that probably function in stress tolerance: water channel proteins involved in the movement of water through membranes, the enzymes required for the biosynthesis of various osmoprotectants (sugars, Pro, and Gly-betaine), proteins that may protect macromolecules and membranes (LEA protein, osmotin, antifreeze protein, chaperon, and mRNA binding proteins), proteases for protein turn over (thiol proteases, Clp protease, and ubiquitin), the detoxification enzymes (glutathione S-transferase, soluble epoxide hydrolase, catalase, superoxide dismutase, and ascorbate peroxidase). Some of the stress-inducible genes that encode proteins, such as a key enzyme for Pro biosynthesis, were over expressed in transgenic plants to produce a stress tolerant phenotype of the plants; this indicates that the gene products really function in stress tolerance ( Shinozaki ,1996 ). The second group contains protein factors involved in further regulation of signal transduction and gene expression that probably function in stress response: Most of the regulatory proteins are involved in signal transduction. Now it becomes more important to elucidate the role of these regulatory proteins for further understanding of plant responses to water deficit. Many transcription factor genes were stress inducible, and various transcriptional regulatory mechanisms may function in regulating drought, cold, or high salinity stress signal transduction pathways. These transcription factors could govern expression of stress-inducible genes either cooperatively or independently, and may constitute gene networks in Arabidopsis ( Pathan.2004 ), 2.5. Model plant for studying the drought tolerant Arabidopsis thaliana is a small weed in the mustard family. It has been a convenient for studies in classical genetics for over forty years ( Redei,1975). This flowering plant also has a genome size and genomic organization that recommend it for certain experiments in molecular genetics and it is coming to be widely used as a model organism in plant molecular genetics, development, physiology, and biochemistry. Arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size and short life cycle. Arabidopsis thaliana, a genetic model plant, has been extensively used for unravelling the molecular basis of stress tolerance. Arabidopsis also proved to be extremely important for assessing functions for individual stress associated genes due to the availability of knock-out mutants and its amenability for genetic transformation. It has been collected or reported in many different regions and climates, ranging from high elevations in the tropics to the cold climate of northern Scandinavia and including locations in Europe, Asia, Africa, Australia, and North America (Kirchheim,1981). Arabidopsis has the smallest known genome among the higher plants. The reasons for a small genome include little repetitive DNA and, in some cases, simpler gene families. Leutwiler et al. (1984) reported that the haploid genome from Arabidopsis (n = 5 chromosomes) contains only roughly 70,000 kilobase pairs (kb). The contrast of the Arabidopsis genome with that of other plants frequently used in molecular genetic work is striking: tobacco, for example, has a haploid nuclear genome of 1,600,000 kb; the pea haploid genome is 4,500,000 kb; and the wheat haploid genome is 5,900,000 kb . The significance of this small DNA content for molecular genetics is that a genomic library of Arabidopsis chromosomal fragments is easy to make, and simple and economical to screen. It is thus rapid and inexpensive to repeatedly screen Arabidopsis genomic libraries. In addition to its remarkably low content of nuclear DNA, Arabidopsis has a genomic organization that makes it uniquely suited to certain ty pes of molecular cloning experiments. All of the properties of the plant small, short generation time, high seed set, ease of growth, self- or cross-fertilization at willmake Arabidopsis a convenient subject for studies in classical genetics. 2.6. Drought related gene Alcohol dehydrogenase and pyruvate decarboxylase are enzyme whose activity has been observed in numerous higher plants including Arabidopsis, maize, pearl millet, sunflower, wheat, and pea (Gottlieb, 1982). In a number of plants, different ADH genes are expressed in various organs, at specific times during development, or in re-sponse to environmental signals. High levels of ADH activity are found in dry seeds and in anaerobically treated seeds (Freeling, 1973. Banuett-Bourrillon .1979), roots (Freeling .1973), and shoots (App, 1958). During periods of anaerobic stress, the enzyme is presumably required by plants for NADH metabolism, via reduction of acetaldehyde to ethanol. With respect to secondary metabolites, ADH is involved in the inter conversion of volatile compounds such as aldehydes and alcohols (Bicsak et al., 1982; Molina et al., 1986; Longhurst et al., 1990). The ethanolic fermentation pathway branches off the main glycolytic pathway at pyruvate. In the first step, pyruvate is the substrate of pyruvate decarboxylase (PDC), yielding CO2 and acetaldehyde. Subsequently, acetaldehyde is reduced to ethanol with the concomitant oxidation of NADH to NAD+ by alcohol dehydrogenase (ADH). Although PDC and ADH gene induction has been demonstrated, ethanol and acetaldehyde production as a result of stress treatment has only been reported for red pine (Pinus resinosa) and birch (Betula spp.) seedlings exposed to sulfur dioxide, water deficiency, freezing, and ozone(Kimmerer and Kozolowski. 1982). Many plants contain more than one ADH gene (Gottlieb, 1982 ), resulting in the expression of different ADH proteins (i.e. ADH isozymes, often designated ADH 1, ADH2, etc. ). The most extensive study of maize Adh genes, AdhI and Adh2, have been cloned and sequenced. The coding sequences of these genes are 82% homologous, interrupted by nine identically positioned introns that differ in sequence and length. The expression of the Arabidopsis Adh gene (Chang and Meyerowitz, 1986; Dolferus et al., 1990) has many features in common with maize Adhl gene (Walker et al., 1987). The two genes have comparable developmental expression pattens, and both have tissue-specific responses to hypoxic stress. In both maize and Arabidopsis, the gene is expressed in seeds, roots, and pollen grains, whereas green aerial plant parts are devoid of detectable levels of ADH activity. In both species, hypoxic induction of the gene occurs in cells of the root system (reviewed by Freeling and Bennett, 1985; Dolferus and Jacobs, 1991; Okimoto et al., 1980;). ADH is induced anaerobically in Arabidopsis (Dolferus, 1985) as in maize. ADH is also induced in both maize root and Arabidopsis callus by the synthetic auxin 2,4-dichlorophenoxyacetic acid (Dolferus,1985. Feeling, 1973). Several approaches have been undertaken to assess the functional role of Adh in development, stress response, and metabolite synthesis. The expression of the alcohol dehydrogenase (Adh) gene is known to be regulated developmentally and to be induced by environmental stresses (Christie et al., 1991; Bucher et al., 1995). Alcohol dehydrogenase (ADH) plays a key enzymatic function in the response to anaerobic conditions in plants (Sachs, Subbaiah, and Saab 1996). A new and exciting aspect of ethanolic fermentation is the suggested involvement in stress signaling and response to environmental stresses other than low oxygen (Tadege et al., 1999). Furthermore, specific analysis of the ADH gene from rice (Oryza sativa), maize, and Arabidopsis showed ADH to be induced by cold (Christie et al., 1991), wounding (Kato-Noguchi, 2001), dehydration (Dolferus et al., 1994), and the phytohormone abscisic acid (ABA; de Bruxelles et al., 1996), in line with the observation from the micro-array experim ents. In Arabidopsis thaliana, Adh overexpression improved the tolerance of hairy roots to low oxygen conditions and was effective in improving root growth (Dennis et al., 2000; Shiao et al., 2002). However, it had no effect on flooding survival (Ismond et al., 2003). Adh over expression in tomato has been shown to modify the balance between Cà ¢Ã¢â¬Å¡Ã¢â¬ , Adh overexpression in tomato aldehydes and alcohols in ripe fruits (Speirs et al., 1998). Grapevine plants overexpressing Adh displayed a lower sucrose content, a higher degree of polymerization of proanthocyanidins, and a generally increased content of volatile compounds, mainly in carotenoid- and shikimate-derived volatiles (Catherine et al., 2006).
Saturday, July 20, 2019
a separte peace :: essays research papers
Literary Analysis for A Separate Peace à à à à à Having a best friend means not to have jealousy of them, and to not wish to hurt them in any way. From reading this literature book, A Separate Peace, by John Knowles, it proves that statement. The story is about two close friends whose bond becomes ruined by jealousy. à à à à à Gene felt extremely jealous of Finny. In the beginning of the story, the author tried to describe the inferior feelings of Gene. In the dorm rooms, Gene tried on Finnyââ¬â¢s clothes as a symbol of wanting to live Finnyââ¬â¢s life. While picking up the shirt, Gene said, ââ¬Å"This is going to be my emblemâ⬠(18). That shows that he wants some artifact of Finnyââ¬â¢s as his label to describe his personality. Basically, he wants to live Finnyââ¬â¢s life.à à à à à Finny was a down-to-earth, normal, peace making guy. While Gene played the role of a follower, Finny played the leader. When Finny tried to beat a swimming record and accomplished it, he wanted it to be kept on the low. Gene, on the other hand, wanted everyone to know therefore he would become popular. After beating the record, Finny said, ââ¬Å"By the way, we arenââ¬â¢t going to talk about this. Itââ¬â¢s just between you and me. Donââ¬â¢t say anything about it, to â⬠¦ anyoneâ⬠(36). By showing that Finny has to say something in the first place, he obviously knows Geneââ¬â¢s blabbering side of him. When Gene pushed Finny off the tree, it clearly showed how jealous he was of him. The boys have such an incredible bond that when Gene came to tell Finny that it was him who pushed him out of the tree, he did not even believe him. He said, ââ¬Å"Of course you didnââ¬â¢t do it. You damn fool. Sit down, you damn fool. Iââ¬â¢m going to hit you if you donââ¬â¢t sit downâ⬠(62). He became so angry and up-tight that Gene would say that, and he truly believed that he would never do anything like that. à à à à à After falling out of a tree, Finny was taken to the hospital. When he felt lonely, he called Gene to talk, and to see if Gene had replaced Finny with another roommate. Once Gene answered ââ¬Å"noâ⬠, Gelber 3 Finny became mad at himself for even thinking that in the first place.
Friday, July 19, 2019
Diabetes Mellitus Essay -- Health Diseases Diabetes Essays medical
Diabetes Mellitus Diabetes is the seventh leading cause of death listed in the United States. Diabetes is the leading cause of blindness. ââ¬Å"In 1996 diabetes contributed to more than 162,000 deathsâ⬠(Lewis 1367). à à à à à ââ¬Å"Diabetes mellitus is not a single disease but a group of disorders with glucose intolerance in commonâ⬠(McCance 674). Diabetes mellitus is a metabolic disorder characterized by hyperglycemia (increased blood sugar) and results from defective insulin production, secretion, and utilization. There are many forms of diabetes. ââ¬Å"Diabetes increases the risk of heart and blood vessel disease, amputation, infections, kidney damage, eye problems (including blindness), and nerve malfunctionâ⬠(Husain). I will briefly define the different forms of diabetes and then I will discuss diabetes mellitus in general. 1.à à à à à Insulin dependent diabetes mellitus (IDDM) or type 1 is when the pancreas will produce little or no insulin, therefore requiring injections of insulin to control diabetes and prevent ketoacidosis. All patients with this type of diabetes need insulin to survive (Deakins 34). Five to ten percent of all diabetic patients have IDDM. Usual presentation is rapid with classical symptoms of polydipsia (increased thirst), polyphagia (increased hunger), polyuria (increased urination), and weight loss. IDDM is most commonly seen in patients under thirty, but can be seen in older adults. 2.à à à à à Non-insulin dependent diabetes mellitus (NIDDM) or type 2 is caused by a defect in insulin manufacture and release from the beta cell or insulin resistance in the peripheral tissues. Approximately ninety percent of diabetic patients have NIDDM. Genetics play a big role in the etiology of NIDDM and is often associated with obesity. Usually presentation is slow and often insidious with symptoms of fatigue, weight gain, poor wound healing, and recurrent infection. Primarily occurs in adults over thirty. 3.à à à à à Gestational diabetes mellitus (GDM) is defined as carbohydrate intolerance, which occurs during pregnancy. Occurs in approximately three percent of pregnancies, and usually disappears after pregnancy. Women with GDM are at higher risk for having diabetes in the future. GDM is associated with increased risk of morbidity. Women should be screened for GDM between the 24th and the 28th weeks of gestation. 4.à à à à à Diabetic ketoacidosis is a serious... ...ur to six hours to reach the bloodstream, but stays in the bloodstream for about thirty-six hours. It peaks about fourteen to twenty-four hours after injection. Diabetes affects the entire body. It slowly deteriorates every one of the bodyââ¬â¢s organs. You can live a long and prosperous life as a well-controlled diabetic, but a short and miserable life if noncompliant. Works Cited Deakins, Dee A. Oral Hypoglycemics. Nursing 92; The Worldââ¬â¢s Largest Nursing Journal Nov. 1992: 34-39. Doenges, Marilynn E. Nursing Care Plans; Guidelines for Planning Patient Care. Philadelphia: F.A. Davis Company, 1989. Drass, Janice. Insulin Injections. Nursing 92; The Worldââ¬â¢s Largest Nursing Journal Nov. 1992:40-43. Harkreader, Helen. Fundamentals of Nursing; Caring and Clinical Judgment. Philadelphia: W.B. Saunders Company, 2000. Husain, Rahat. The Diabetes Information Page. Undated. http://www.geocities.com/Athens/Forum/5769/diabete.html. Lewis, Sharon Mantik. Medical Surgical Nursing: Assessment and Management of Clinical Problems. St. Louis: Mosby, 2000. McCance, Kathryn L. Pathophysiology; The Biologic Basis for Disease in Adults and Children. St. Louis: Mosby, 1994.
A Comparison And Contrast In Both As Worn By Hester And Dimmesdale :: essays research papers
A Comparison and Contrast In Both A's Worn By Hester and Dimmesdale The two A's worn in the novel by both Hester and Dimmesdale are dramatically different, yet they are born and made by the same identical sins. These letters are also differentiated by the infinitely changing emotional state and physical well being of the character, the towns views of morality and natural order, and the affecting environment. The two sins of most importance in the novel and that serve the greatest beneficiality in the appearance of the A's are--of course-- adultery and hypocrisy. The separation in the appearance of both of the A's begins with each characters own personal interpretation of the extremity of their sins. Where Hester's A is beautiful and artistically done ("fantastically embroidered and illuminated upon her bosom; pg.37) her interpretation of the extremity of her sins is one of self composure and nonchalantness. She views her sins solely as a "violation in the natural order" of the environment and therefore cannot even perceive her sin as being evil except through outside brainwashing. While Dimmesdale's personal interpretation as to the extremity of his own sins is a "violation of God's law," which is the law that he is totally dedicated to and supported by. Dimmesdale's interpretation of his sin is much more severe than Hester's, it is a breach and direct contradiction of his own self consciousness and physical existence. Therefore the appearance of his A, even though it is never directly described in the novel, must be raw, jagged, and brutally crooked (...a ghastly rapture; pg.95). Maybe Dimmesdale's self torture is so horrifying or inconceivable that it is either indescribable, (...too mighty to be expressed only by the eye of his figure; pg.95), or best left up to the reader's imagination. Unlike Hester, Dimmesdale, because of self interpretation, cannot in any way conceive his sins of being anything but evil. Although the appearance of the A's are proportional to the interpretation by each character; also the appearance of the A's is directly correlated between the consequences each character receives because of their sins, both Hester's and Dimmesdale's punishment is introduced through a new character and some sort of isolation. The new character's are a form of abstract contrasting where each new character is an extension of the sinner's "A" itself. Where as Chillingworth is a doubled extension of Dimmesdale's consciousness; Pearl is a contrast to Hester's creativity, patience, and composure. Dimmesdale's punishment through Chillingworth is one of mental bombardment and spiritual torture which supports the theory that Dimmesdale's A must be horrifically putrid and indescribable. Pearl's punishment towards
Subscribe to:
Posts (Atom)